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KMID : 0613820120220070889
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Journal of Life Science
2012 Volume.22 No. 7 p.889 ~ p.896
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Whitening Effect of Hizikia fusiformis Ethanol Extract and Its Fractions
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Jeon Myong-Je
Kim Mi-Hyang
Jang Hye-Ji
Lee Seung-Woo
Kim Jae-Hoon
Kim Hyung-Suk
Lee Sang-Hyeon
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Abstract
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Melanin synthesis is catalyzed by tyrosinase. To investigate the whitening effect of Hizikia fusiformis, fractions from ethanol extract of H. fusiformis were prepared by a systematic fractionation procedure with solvents such as methanol, hexane, butanol, and H₂O₂. The ethanol extract and its fractions were then subjected to evaluate the inhibitory effects on the tyrosinase activity and melanin synthesis in murine B16F10 melanoma cells. The ethanol extract and aqueous fraction exhibited a whitening effect with no cytotoxicity. The ethanol extract showed the highest whitening effect among the samples. The inhibitory effect of 100μg/ml of ethanol extract was higher than that of 10μg/ml of arbutin, but it was lower than that of 10μg/ml of kojic acid. Furthermore, the inhibitory effects of 100μg/ml of methanol, hexane, butanol, and aqueous fractions were similar to those of 10μg/ml of arbutin. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. The ethanol extract and aqueous fraction showed relatively higher DPPH radical-scavenging activities compared with the other samples. Furthermore, 500μg/ml of ethanol extract and aqueous fraction diminished LPS-induced iNOS expression to 82 and 80%, respectively. These results suggest that ethanol extract and aqueous fraction of H. fusiformis could be used as cosmetic ingredients for whitening and skin protection effects.
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KEYWORD
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Hizikia fusiformis, melanin, tyrosinase activity, whitening effect
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